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Construction of fusion vectors of corynebacteria:expression of glutathione-S-transferase fusion protein in Corynebacterium acetoacidophilum ATCC 21476

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Author: Srivastava, Preeti; Deb, J K

Advisor: Advisor

Date: 2002

Publisher:
Citation: FEMS Micro

Series/Report no.:
Item Type: Article

Keywords: Corynebacterium; Glutathione-S-transferase fusion vector; Enhanced green fluorescent protein; Streptokinase

Abstract: A series of fusion vectors containing glutathione-S-transferase (GST) were constructed by inserting GST fusion cassette of Escherichia coli vectors pGEX4T-1,-2 and -3 in corynebacterial vector pBK2. Efficient expression of GST driven by inducible tac promoter of E. coli was observed in Corynebacterium acetoacidophilum. Fusion of enhanced green fluorescent protein (EGFP) and streptokinase genes in this vector resulted in the synthesis of both the fusion proteins. The ability of this recombinant organism to produce several-fold more of the product in the extracellular medium than in the intracellular space would make this system quite attractive as far as the downstream processing of the product is concerned.
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Shankar B. Chavan
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shankar.chavan@library.iitd.ac.in
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etd@IISc
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Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
NewsClips @IITD
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