| Author: | Patwardhan, P R; Srivastava, A K |
| Advisor: | Advisor |
| Date: | 2004
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| Publisher: | |
| Citation: | Biochemica
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| Series/Report no.: |
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| Item Type: | Article
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| Keywords: | PHB; Modelling; Model-based fed-batch cultivation |
| Abstract: | Batch kinetics of the PHB synthesiswas established in bioreactor under controlled environmental conditions of pH and issolved oxygen. The data of the batch kinetics was used to develop and identify a mathematical model. The nutrient feeding strategies in the fed-batch cultivation were developed aiming at maintenance of limiting nutrient for either growth enhancement or increased intracellular product accumulation by offline computer simulation of mathematical model. The selected fed-batch fermentation strategies, using the predicted feeding profiles, were experimentally implemented to improve the growth/product formation/productivity and establish the validity of the developed model. The model predictions were very close to the observed experimental data points. |
