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Purification and characterization of two B-glucosidases from a thermo-tolerant yeast pichia etchellsii

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Author: Wallecha, Anu; Mishra, Saroj

Advisor: Advisor

Date: 2003

Publisher:
Citation: Biochimica

Series/Report no.:
Item Type: Article

Keywords: B-Glucosidase; Pichia etchellsii; Alkyl-glucoside; Glycosyl transferase activity

Abstract: The thermo-tolerant yeast Pichia etchellsii produced two cell-wall-bound inducible B-glucosidases, BGLI (molecular mass 186 kDa) and BGLII (molecular mass 340 kDa), which were purified by a simple, three-step method, comprising ammonium sulfate precipitation, ionexchange and hydroxyapatite chromatography. The two enzymes exhibited a similar pH and temperature optima, inhibitory effect by glucose and gluconolactone, and stability in the pH range of 3.0–9.0. Placed in family 3 of glycosylhydrolase families, BGLI was more active on salicin, p-nitrophenyl B-D-glucopyranoside and alkyl B-D-glucosides whereas BGLII was most active on cellobiose. kcat and KM values were determined for a number of substrates and, for BGLI, it was established that the deglycosylation step was equally effective on aryl- and alkylglucosides while the glycosylation step varied depending on the substrate used. This information was used to synthesize alkyl-glucosides (up to a chain length of C10) using dimethyl sulfoxide stabilized single-phase reaction microenvironment. About 12% molar yield of octylglucoside was calculated based on a simple spectrophotometric method developed for its estimation. Further, detailed comparison of properties of the enzymes indicated these to be different from the previously cloned B-glucosidases from this yeast.
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Shankar B. Chavan
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Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
NewsClips @IITD
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