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A continuous process for production of d-ribulose-1,5-bisphosphate from d-glucose

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Author: Bhattacharya, Sumana; Nayaka, Amiya; Gomes, James; Bhattacharya, Sanjoy K

Advisor: Advisor

Date: 2004

Publisher:
Citation: Biochemica

Series/Report no.:
Item Type: Article

Keywords: RuBP production; Bioreactors; Carbon dioxide fixation start-up; Immobilized enzymes

Abstract: A continuous bioprocess for production of d-ribulose-1,5-bisphosphate (RuBP) using d-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely,hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from d-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.
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Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
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