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κ-Carrageenan as a carrier in affinity precipitation of yeast alcohol dehydrogenase

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Author: Mondal, Kalyani; Roy, Ipsita; Gupta, Munishwar N

Advisor: Advisor

Date: 2003

Publisher:
Citation: Protein Ex

Series/Report no.:
Item Type: Article

Keywords: Affinity precipitation; Alcohol dehydrogenase; κ-Carrageenan; Reversibly soluble–insoluble polymers; Protein purification

Abstract: κ-Carrageenan is a non-toxic polymer from seaweeds, which becomes reversibly insoluble upon the addition of K+. Its conjugate with the dye, Cibacron Blue 3GA, was used to purify alcohol dehydrogenase from crude yeast extract by affinity precipitation. Response surface methodology was used to optimize conditions for affinity precipitation of the enzyme with the polymer–dye conjugate. Recovery of 58% of the enzyme activity with 13.6-fold purification was obtained.
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Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
NewsClips @IITD
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