κ-Carrageenan as a carrier in affinity precipitation of yeast alcohol dehydrogenase

dc.contributor.author	Mondal, Kalyani
dc.contributor.author	Roy, Ipsita
dc.contributor.author	Gupta, Munishwar N
dc.date.accessioned	2006-01-24T05:57:10Z
dc.date.accessioned	2019-02-10T14:23:51Z
dc.date.accessioned	2019-02-11T06:18:56Z
dc.date.available	2006-01-24T05:57:10Z
dc.date.available	2019-02-10T14:23:51Z
dc.date.available	2019-02-11T06:18:56Z
dc.date.issued	2003
dc.identifier.citation	Protein Expression and Purification, 32(1), 151-160	en
dc.identifier.uri	http://localhost:8080/iit/handle/2074/1233
dc.description.abstract	κ-Carrageenan is a non-toxic polymer from seaweeds, which becomes reversibly insoluble upon the addition of K+. Its conjugate with the dye, Cibacron Blue 3GA, was used to purify alcohol dehydrogenase from crude yeast extract by affinity precipitation. Response surface methodology was used to optimize conditions for affinity precipitation of the enzyme with the polymer–dye conjugate. Recovery of 58% of the enzyme activity with 13.6-fold purification was obtained.	en
dc.format.extent	489584 bytes
dc.format.mimetype	application/pdf
dc.language.iso	en	en
dc.subject	Affinity precipitation	en
dc.subject	Alcohol dehydrogenase	en
dc.subject	κ-Carrageenan	en
dc.subject	Reversibly soluble–insoluble polymers	en
dc.subject	Protein purification	en
dc.title	κ-Carrageenan as a carrier in affinity precipitation of yeast alcohol dehydrogenase	en
dc.type	Article	en