Browsing Faculty Research Publications by Author "Eshwari, A N S"
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Optimization of immobilized metal ion affinity chromatography for single-step purification of recombinant ovine growth hormone expressed in escherichia coli
Gupta, V; Eshwari, A N S; Panda, A K; Agarwal, G P (2003)In the present investigation, Sepharose 6B gel with 1,4-butanediol diglycidyl ether as spacer arm, iminodiacetic acid as the 21 21 ligand and Cu , Ni as metal ions were used to prepare an immobilized metal ion affinity ...