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Immobilization of trypsin on an enteric polymer Eudragit S-100 for the biocatalysis of macromolecular substrate
Trypsin was covalently linked to Eudragit S-100 an enteric coating polymer by carbodiimide coupling method. Nearly 90% of enzyme activity was conjugated to the polymer whereas actual observed expressed activity was 64%. ...
Noncovalent immobilization of enzymes on an enteric polymer eudragit S-100
The noncovalent immobilization of enzymes such as alpha-amylase, beta-glucosidase, trypsin, and alkaline phosphatase was performed by adsorption on the water-soluble polymer Eudragit S-100. The strength of the binding with ...
Affinity precipitation of trypsin with soybean trypsin inhibitor linked Eudragit S-100
Soybean trypsin inhibitor linked to Eudragit S-100 was used for the affinity precipitation of trypsin. Polymer and ligand concentrations used in conjugate preparation showed remarkable effect on the trypsin recovery. Trypsin ...