Now showing items 1-6 of 6
Purification of phospholipase D by two-phase affinity extraction
An aqueous two-phase system of polyethylene glycol (PEG)–salt was used for purification of phospholipase D (PLD) from peanuts and carrots. Alginate, a known macroaffinity ligand for PLD, was incorporated in the PEG phase ...
Affinity precipitation of aspergillus niger pectinase by microwave-treated alginate
Affinity precipitation is a simple, single plate separation process in which the complex of a smart macroaffinity ligand with the target protein (from a crude broth) can be selectively precipitated by application of a ...
Separation of phospholipase D from peanut on a fluidized bed of crosslinked alginate beads
Alginate, a polysaccharide consisting of guluronic acid and mannuronic acid residues, has been crosslinked with epichlorohydrin to obtain beads. The crosslinked alginate beads were used in a fliudized bed to purify ...
Three phase partitioning of carbohydrate polymers: separation and purification of alginates
Three phase partitioning, a technique described for protein purification, has been employed for precipitation and purification of three different commercial preparations of alginates. Three phase partitioning works by the ...
An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate
B-amylases are used in production of maltose syrup. It is shown that sweet potato B-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified ...
Magnetic alginate microparticles for purification of α-amylases
Spherical magnetic alginate microparticles (25–60 μm in diameter) were prepared using the microemulsion system, with water-saturated 1-pentanol as the organic phase. The limited solubility of 1-pentanol in water enabled ...