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Purification of a bacterial pullulanase on a fluidized bed of calcium alginate beads
Pullulanase from Bacillus acidopullulyticus was purified on a packed bed and a fluidized bed of calcium alginate beads. The binding of enzyme activity to the medium was found to follow Langmuir isotherm pattern. The maximum ...
κ-Carrageenan as a new smart macroaffinity ligand for the purification of pullulanase
κ-Carrageenan is a polysaccharide from red seaweed which gets precipitated by K+ ions and dissolves again in water. This smart, K+-responsive polymer was found to selectively bind pullulanase activity from Bacillus ...
Preparation of highly active α-chymotrypsin for catalysis in organic media
A simple one step process for the preparation of free α-chymotrypsin, using an organic solvent to precipitate the enzyme from a buffered solution, followed by washing with organic solvents, is described. This preparation ...