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Reversibly soluble macroaffinity ligand in aqueous two-phase separation of enzymes
Use of alginate as a free bioligand incorporated in an aqueous two-phase system of polyethylene glycol 6000–salt resulted in considerable purification of wheat germ α-amylase and sweet potato β-amylase from their crude ...
Purification of phospholipase D by two-phase affinity extraction
An aqueous two-phase system of polyethylene glycol (PEG)–salt was used for purification of phospholipase D (PLD) from peanuts and carrots. Alginate, a known macroaffinity ligand for PLD, was incorporated in the PEG phase ...