Biochemical Engg. and Biotechnology
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Process engineering studies on continuous ethanol production by immobilized S. cerevisiae
(1992)Saccharomyces cerevisiae cells were immobilized on three supports—calcium alginate beads, raw and hexamethylene diamine treated bagasse—for their comparative assesment in terms of cell retention on to the carrier and ... -
Physiological factors of growth and susceptibility to virus regulating Vero cells for optimum yield of vaccinia and cloned gene product (β-hCG)
(1994)Replication of vaccinia virus carrying β-hCG gene at the ‘tk’ locus was studied in microcarrier culture of Vero cell. The yields of recombinant vaccinia virus and its expressed product (β-hCG) were influenced by the ... -
Cascade of bioreactors in series for conversion of 3-phospho-d-glycerate into d-ribulose-1,5-bisphosphate: kinetic parameters of enzymes and operation variables
(2004)A novel scheme employing enzymatic catalysts is described enabling conversion of d-ribulose-1,5-bisphosphate (RuBP) from 3-phospho-d-glycerate (3-PGA) without loss of carbon. Bioreactors harboring immobilized enzymes namely, ... -
A continuous process for production of d-ribulose-1,5-bisphosphate from d-glucose
(2004)A continuous bioprocess for production of d-ribulose-1,5-bisphosphate (RuBP) using d-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow ... -
Catalysis by ATP synthase: mechanistic, kinetic and theromodynamic characteristics
(2001)Mechanistic,kinetic and thermodynamic aspects of ATP synthase have been determined and analyzed.Reversibility and inversibility of catalysis in ATP synthasis represent two contrasting modes of catalysis with important ... -
Interactions of proteins with immobilized metal ions
(2001)The design, optimization, and scale-up of a chromatographic process using immobilized metal ion affinity chromatography (IMAC)demands a thorough understanding of the fundamental factors governing the various interactions ... -
Purification and characterization of two B-glucosidases from a thermo-tolerant yeast pichia etchellsii
(2003)The thermo-tolerant yeast Pichia etchellsii produced two cell-wall-bound inducible B-glucosidases, BGLI (molecular mass 186 kDa) and BGLII (molecular mass 340 kDa), which were purified by a simple, three-step method, ... -
Studies on protein transmission in thin channel flow module:the role of dean vortices for improving mass transfer
(2002)This study is mainly comprised of evaluating mass transfer coefficient from the volumetric flux and protein transmission data in a thin channel flow module. Dilute solutions of lysozyme were ultrafiltered through hydrophilic ... -
Recent advances in microbial polyhydroxyalkanoates
(2004)Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates (HAs) synthesised by numerous bacteria as an intracellular carbon and energy storage compound. These are accumulated in the cytoplasm of cells. A number of ... -
Improvement of gibberellic acid production using a model based fed-batch cultivation of gibberella fujikuroi
(2004)The production of gibberellic acid (GA3) by submerged cultivation of Gibberella fujikuroi was carried out in a 3l fermenter. Under optimum culture conditions, the batch culture exhibited a maximum biomass concentration of ... -
Factors limiting bacterial iron oxidation in biodesulphurization system
(2004)Batch studies have been conducted to examine the effect of various factors (which are suspected to limit the coal biodesulphurization process rate) on bacterial oxidation of ferrous iron. An attempt has been made to find ... -
Model-based fed-batch cultivation of R. eutropha for enhanced biopolymer production
(2004)Batch kinetics of the PHB synthesiswas established in bioreactor under controlled environmental conditions of pH and issolved oxygen. The data of the batch kinetics was used to develop and identify a mathematical model. ... -
Utilisation of fruits waste for citric acid production by solid state fermentation
(2003)A solid state fermentation method was used to utilise pineapple, mixed fruit and maosmi waste as substrates for citric acid production using Aspergillus niger DS 1. Experiments were carried out in the presence and absence ... -
Statistical media optimization and alkaline protease production from bacillus mojavensis in a bioreactor
(2003)Alkaline protease production in Bacillus mojavensis was improved up to 4.2-fold in a 14 l bioreactor during validation of a predicted statistical model. The final enzyme yield in the bioreactor was 2389 U m1 obtained within ... -
Microbicidal male contraceptive - risug induced morphostructural damage in e.coli
(2003)Risug, a new polymeric drug with antimicrobial properties has been shown to alter the cell morphology and structure of Escherichia coli.Topographic images, traces of surface topography and analysis of surface roughness ... -
Citric acid production by solid state fermentation using sugarcane bagasse
(2003)A solid state fermentation (SSF) method was used to produce citric acid by Aspergillus niger DS 1 using sugarcane bagasse as a carrier and sucrose or molasses based medium as a moistening agent. Initially bagasse and wheat ... -
Optimization of immobilized metal ion affinity chromatography for single-step purification of recombinant ovine growth hormone expressed in escherichia coli
(2003)In the present investigation, Sepharose 6B gel with 1,4-butanediol diglycidyl ether as spacer arm, iminodiacetic acid as the 21 21 ligand and Cu , Ni as metal ions were used to prepare an immobilized metal ion affinity ... -
Partial characterization of small plasmids from corynebacterium renale
(1995)A naphthalene-degrading strain of corynebacteria, Corynebacterium renale, harbors multiple small plasmids designated pCR1, pCR2, pCR3, and pCR4 with sizes of 1.4, 3.2, 4.4, and 5.7 kb, respectively. Plasmid pCR1 of 1.4 kb ... -
Production of methionine by a multi-analogue resistant mutant of corynebacterium lilium
(2003)Multi-analogue resistant mutants of Corynebacterium lilium were developed using UV and N-methyl-N,-nitro-nitrosoguanidine (NTG) mutagenesis in order to overproduce methionine. Although the methionine yield at each stage ... -
A potential resource for bioconversion of domestic wastewater sludge
(2002)Twenty seven filamentous fungal strains representing five genera; Aspergillus, Penicillium, Trichoderma, Myriodontium and Pleurotus were isolated from four sources; domestic wastewater sludge cake (SC) from IWK (Indah Water ... -
Optimization of compactin production in chemically defined production medium by penicillium citrinum using statistical methods
(2002)Production of compactin by Penicillium citrinum was optimized using Plackett-Burman and central composite rotatable design.Out of 11 factors studied by Plackett-Burman design, six influenced the compactin production. Central ... -
The torsional mechanism of energy transduction and ATP synthesis as a breakthrough in our understanding of the mechanistic, kinetic and thermodynamic details
(2004)Novel insights into mechanistic, kinetic and thermodynamic details of ATP synthesis by F1F0-ATP synthase in oxidative phosphorylation and photophosphorylation have been offered from the perspective of the torsional mechanism ... -
Adsorption properties of the fibril forming protein from trichoderma reesei
(2002)The amino acid composition and adsorption properties of the fibril forming protein (FFP), identified previously as a non-hydrolytic component of Trichoderma reseei cellulase enzymes, are described. The amino acid composition ... -
The detailed molecular mechanism of ATP synthesis in the F0 portion of ATP synthase reveals a non-chemiosmotic mode of energy coupling
(2002)Based on our torsional mechanism of ion translocation, energy transduction and energy storage in ATP synthase [Curr. Sci. 75(1998) 716]; [Curr. Sci. 77 (1999) 167]; [Curr. Sci. 78 (2000) 23]; [Biochem. Biophys. Res. Commun. ... -
Construction of fusion vectors of corynebacteria:expression of glutathione-S-transferase fusion protein in Corynebacterium acetoacidophilum ATCC 21476
(2002)A series of fusion vectors containing glutathione-S-transferase (GST) were constructed by inserting GST fusion cassette of Escherichia coli vectors pGEX4T-1,-2 and -3 in corynebacterial vector pBK2. Efficient expression ... -
Productivity improvement in l-sorbose biosynthesis fedbatch cultivation of gluconobacter oxydans
(2002)The effect of increased (100, 200 and 300 gl) initial sorbitol concentrations (S) was investi-gated in the sorbitol to sorbose bioconversion process. Batch cultivations with a S of 100,200 and 300 g1 were completed at 10, ... -
Role of water structure on phase separation in polyelectrolyte-polyethyleneglycol based aqueous two phase systems
(2002)Partitioning of proteins in aqueous two-phase system(ATPS)has emerged as one of the important downstream processing techneques in bioprocess technology.The phase separation behavior of polyelectrolyte-polythyleneglycol(PEG)based ... -
Effect of seed culture on solid-state bioconversion of wheat straw by phanerochaete chrysosporium for animal feed production
(2002)The solid-state bioconversion of wheat straw by Phanerochaete chtysosporium for the produc-of animal feed was studied. This study was performed based on central composite experimental design.The conditions of the seed ... -
Production of podophyllotoxin by plant cell cultures of podophyllum hexandrum in bioreactor
(2002)Submerged cultivation of Podophyllum hexandrum for the production of podophyllotoxin was carried out in a 3I stirred tank bioreactor fitted with a low-shear Setric impeller. The specific re- quirements of the medium, such ...
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