Browsing Biochemical Engg. and Biotechnology by Title
Now showing items 8-27 of 73
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Biodesulphurization of coal: effect of pulse feeding and leachate recycle
(2001)Biodesulphurization of coal was carried out under four modes of operation namely: conventional batch, constant volume pulse feeding (CVPF), increasing volume pulse feeding (IVPF) and leachate recycle. The effects of different ... -
Biodesulphurization of Indian (Assam) coal using thiobacillus ferrooxidans (ATCC 13984)
(2000)The pyritic sulphur removal from coal and pyrite by Thiobacillus ferrooxidans was studied in a batch reactor. Microbial oxidation of ferrous iron to the ferric form, the central step in the biodesulphurization process was ... -
Biosynthesis of tannase and hydrolysis of tannins to gallic acid by aspergillus awamori — optimisation of process parameters
(2000)Tannin acyl hydrolase (tannase) is an industrially important enzyme produced by a large number of fungi. Tannic acid concentration, agitation speed and pH during the fermentation were identified as important process ... -
Biosynthesis of xylanolytic and xylan-debranching enzymes in Melanocarpus albomyces IIS 68
(1997)Melanocarpus albomyces IIS 68 synthesizes xylanase, ,&xylosidase and xylan debranching enzymes, namely, acetyl esterase, a+arabinofuranosidase and a-o-giucuronidase, when grown on various substrates. All five enzymes were ... -
Cascade of bioreactors in series for conversion of 3-phospho-d-glycerate into d-ribulose-1,5-bisphosphate: kinetic parameters of enzymes and operation variables
(2004)A novel scheme employing enzymatic catalysts is described enabling conversion of d-ribulose-1,5-bisphosphate (RuBP) from 3-phospho-d-glycerate (3-PGA) without loss of carbon. Bioreactors harboring immobilized enzymes namely, ... -
Catalysis by ATP synthase: mechanistic, kinetic and theromodynamic characteristics
(2001)Mechanistic,kinetic and thermodynamic aspects of ATP synthase have been determined and analyzed.Reversibility and inversibility of catalysis in ATP synthasis represent two contrasting modes of catalysis with important ... -
Citric acid production by solid state fermentation using sugarcane bagasse
(2003)A solid state fermentation (SSF) method was used to produce citric acid by Aspergillus niger DS 1 using sugarcane bagasse as a carrier and sucrose or molasses based medium as a moistening agent. Initially bagasse and wheat ... -
Cloning and Expression of P-Glucosidase Gene from the Yeast Pichia etchellsii
(1995)umerical Modelling of Coastal Upwelling In The Bay of Bengal Pergamon,21,(5),667-670 A 4%kilobase pairs DNA fragment from thermophilic yeast Pichia etchellsii was cloned into the vector plasmid pUC19 to form plasmid pBG55 ... -
Computer coupled substrate feeding strategies for efficient conversion of D-sorbitol to L-sorbose in fed-batch culture
(2001)L-sorbose fermentation is of industrial importance since it is an intermediate step in the industrial production of vitamin C. It involves the biological oxidation of D-sorbitol to L-sorbose using Acetobacter suboxydans. ... -
Construction of fusion vectors of corynebacteria:expression of glutathione-S-transferase fusion protein in Corynebacterium acetoacidophilum ATCC 21476
(2002)A series of fusion vectors containing glutathione-S-transferase (GST) were constructed by inserting GST fusion cassette of Escherichia coli vectors pGEX4T-1,-2 and -3 in corynebacterial vector pBK2. Efficient expression ... -
A continuous process for production of d-ribulose-1,5-bisphosphate from d-glucose
(2004)A continuous bioprocess for production of d-ribulose-1,5-bisphosphate (RuBP) using d-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow ... -
The detailed molecular mechanism of ATP synthesis in the F0 portion of ATP synthase reveals a non-chemiosmotic mode of energy coupling
(2002)Based on our torsional mechanism of ion translocation, energy transduction and energy storage in ATP synthase [Curr. Sci. 75(1998) 716]; [Curr. Sci. 77 (1999) 167]; [Curr. Sci. 78 (2000) 23]; [Biochem. Biophys. Res. Commun. ... -
Effect of cysteine on methionine production by a regulatory mutant of corynebacterium lilium
(2005)The production of methionine by submerged fermentation using a mutant strain of Corynebacterium lilium was studied to determine suitable conditions for obtaining high productivity. The mutant strain resistant to the ... -
Effect of seed culture on solid-state bioconversion of wheat straw by phanerochaete chrysosporium for animal feed production
(2002)The solid-state bioconversion of wheat straw by Phanerochaete chtysosporium for the produc-of animal feed was studied. This study was performed based on central composite experimental design.The conditions of the seed ... -
Effect of sulfur concentration on sludge acidification during the ssdml process
(1996)Heavy metals present in sewage sludges can be leached out, among other processes, by a process using a consortium of sulfur oxidizing bacteria. The metal leaching could be combined with aerobic sludge digestion to result ... -
Effects of dissolved oxygen and oxygen mass transfer on overexpression of target gene in recombinant E. coli
(1997)Overexpression of target gene (Mspl methylase) in recombinant Escherichia coli has been studied in response to dissolved oxygen and oxygen mass transfer. The rate of target gene expression attained its maximum value (2.67 ... -
Enhanced expression of the recombinant lethal factor of bacillus anthracis by fed-batch culture
(2001)High cell density cultivation has been one of the most effective ways to increase cell as well as the product yields. The structural gene for the 90-kDa lethal factor (LF) isolated from Bacillus anthracis was expressed as ... -
Expression and characterization of Pichia etchellsii B-glucosidase in Escherichia coli
(1997)The B-glucosidase enzyme is important as the terminal enzyme involved in hydrolysis of cellobiose and short-chain cellodextrins generated during enzymatic cellulose degradation. Under controlled reaction conditions the ... -
Factors limiting bacterial iron oxidation in biodesulphurization system
(2004)Batch studies have been conducted to examine the effect of various factors (which are suspected to limit the coal biodesulphurization process rate) on bacterial oxidation of ferrous iron. An attempt has been made to find ... -
Fed-batch propionic acid production by propionibacterium acidipropionici
(2000)The batch fermentations were conducted using lactose as the substrate at pH 6.5 and temperature 30C. Average batch kinetic data was eventually used to develop an unstructured mathematical model. The kinetic parameters of ...