Browsing Biochemical Engg. and Biotechnology by Title
Now showing items 28-47 of 73
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Immobilization of trypsin on an enteric polymer Eudragit S-100 for the biocatalysis of macromolecular substrate
(1998)Trypsin was covalently linked to Eudragit S-100 an enteric coating polymer by carbodiimide coupling method. Nearly 90% of enzyme activity was conjugated to the polymer whereas actual observed expressed activity was 64%. ... -
Improvement of gibberellic acid production using a model based fed-batch cultivation of gibberella fujikuroi
(2004)The production of gibberellic acid (GA3) by submerged cultivation of Gibberella fujikuroi was carried out in a 3l fermenter. Under optimum culture conditions, the batch culture exhibited a maximum biomass concentration of ... -
Interactions of proteins with immobilized metal ions
(2001)The design, optimization, and scale-up of a chromatographic process using immobilized metal ion affinity chromatography (IMAC)demands a thorough understanding of the fundamental factors governing the various interactions ... -
Interactions of proteins with immobilized metal ions:a comparative analysis using various isotherm models
(2001)Immobilized metal ion affinity chromatography (IMAC)is now a widely accepted technique for the purification of natural and recombinant therapeutic products and is beginning to find industrial applications. The design,optimization, ... -
Intigrated technique for pyritic sulphur reduction in indian (ASSAM) coal
(1998)Microbial desulphurization of coal using a pyrite oxidizing organism such as thiobacllus fer rooxidans result in approximately 90% pytiric sulphur reduction in 30d. An integrated technique comprised in bacterial preconditioning ... -
An investigation on glycerol biosynthesis by an osmophilic yeast in a bioreactor
(2001)Glycerol production by osmophilic yeasts has a significant potential for large-scale production. The effects of important parameters including initial glucose concentration, carbon/nitrogen (C/N) ratio, aeration rate and ... -
Kinetic model of ATP synthase: pH dependence of the rate of ATP synthesis
(2000)Abstract Recently, a novel molecular mechanism of torque generation in the F0 portion of ATP synthase was proposed [Rohatgi, Saha and Nath (1998) Curr. Sci. 75, 716-718].In this mechanism, rotation of the c-subunit was ... -
Kinetic modeling of ATP synthesis by ATP synthase and Its mechanistic implications
(2000)Based on the torsional mechanism of ATP synthesis by ATP synthase, a kinetic scheme has been developed in this work. The scheme considers adenine nucleotide transport, binding of substrates ADP and Pi, unbinding of product ... -
Microbicidal male contraceptive - risug induced morphostructural damage in e.coli
(2003)Risug, a new polymeric drug with antimicrobial properties has been shown to alter the cell morphology and structure of Escherichia coli.Topographic images, traces of surface topography and analysis of surface roughness ... -
Model-based fed-batch cultivation of R. eutropha for enhanced biopolymer production
(2004)Batch kinetics of the PHB synthesiswas established in bioreactor under controlled environmental conditions of pH and issolved oxygen. The data of the batch kinetics was used to develop and identify a mathematical model. ... -
Modeling and simulation of anaerobic startfied biofilm for mathane production and prediction of multiple steady states
(1997)The present work deal with the modeling and anaerobic metabolism in a startified biofilm consisting of two distinct layers of microorganisms in a fixed film reactor.The model is applied to steady state conditions and its ... -
Modelling the effects of electrostatic interaction with reaction-generated pH change on the kinetics of immobilized enzymes
(1995)A Mathematical model to describe the effects of electrostatic interaction with reaction-generated pH change on the kinetics of immobilized enzyme has been developed to evaluate quantitatively the effect of partitioning of ... -
Necessity of a two-stage process for the production of azadirachtin-related limonoids in suspension cultures of azadirachta indica
(2003)The effect of major nutrients on growth and azadirachtin-related limonoids (AZRL) production in plant cell culture of Azadirachta in&a (neem) was studied with the objective to increase the yield of AZRL, one of the major ... -
A new approach to evaluate kinetic parameters and mass transfer coefficient in continuous stirred tank reactors.Application to antibiotic separation
(1997)A method is proposed to evaluate kinetic parameters and mass transfer cofficients for adsorption processes carried out in continuous stirred tank reactors.This method,employing a biphasic model,does not linerize nonliner ... -
A new approach to evaluate kinetic parameters and mass transfer coefficients stirred tank reactors.Application to antibiotic separation
(1997)A new method is proposed to evaluate kinetic parameters and mass transfer cofficient for adsorption processes carried out in continuous stirred tank reactors.This method,employing a biphasic mode,does not lincarize nonlinear ... -
Optimization of compactin production in chemically defined production medium by penicillium citrinum using statistical methods
(2002)Production of compactin by Penicillium citrinum was optimized using Plackett-Burman and central composite rotatable design.Out of 11 factors studied by Plackett-Burman design, six influenced the compactin production. Central ... -
Optimization of immobilized metal ion affinity chromatography for single-step purification of recombinant ovine growth hormone expressed in escherichia coli
(2003)In the present investigation, Sepharose 6B gel with 1,4-butanediol diglycidyl ether as spacer arm, iminodiacetic acid as the 21 21 ligand and Cu , Ni as metal ions were used to prepare an immobilized metal ion affinity ... -
Optimization of xylanase production by melanocarpus albomyces IIS68 in solid state fermentation using response surface methodology
(2001)Xylanase production by the thermophilic fungus, Melanocarpus albomyces IIS68, during solid state fermentation of wheat straw was studied and the effects of various variables were observed. Using the response surface ... -
Partial characterization of small plasmids from corynebacterium renale
(1995)A naphthalene-degrading strain of corynebacteria, Corynebacterium renale, harbors multiple small plasmids designated pCR1, pCR2, pCR3, and pCR4 with sizes of 1.4, 3.2, 4.4, and 5.7 kb, respectively. Plasmid pCR1 of 1.4 kb ...