Browsing Biochemical Engg. and Biotechnology by Title
Now showing items 47-66 of 73
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Partial characterization of small plasmids from corynebacterium renale
(1995)A naphthalene-degrading strain of corynebacteria, Corynebacterium renale, harbors multiple small plasmids designated pCR1, pCR2, pCR3, and pCR4 with sizes of 1.4, 3.2, 4.4, and 5.7 kb, respectively. Plasmid pCR1 of 1.4 kb ... -
Physiological factors of growth and susceptibility to virus regulating Vero cells for optimum yield of vaccinia and cloned gene product (β-hCG)
(1994)Replication of vaccinia virus carrying β-hCG gene at the ‘tk’ locus was studied in microcarrier culture of Vero cell. The yields of recombinant vaccinia virus and its expressed product (β-hCG) were influenced by the ... -
Plasmids of corynebacteria
(1999)Corynebacteria are pleomorphic, asporogenous, Gram-positive bacteria. Included in this group are nonpathogenic soil corynebacteria, which are widely used for the industrial production of amino acids and detergents, and in ... -
A potential resource for bioconversion of domestic wastewater sludge
(2002)Twenty seven filamentous fungal strains representing five genera; Aspergillus, Penicillium, Trichoderma, Myriodontium and Pleurotus were isolated from four sources; domestic wastewater sludge cake (SC) from IWK (Indah Water ... -
Precise control of dissolved oxygen in bioreactors - a model-based geometric algorithm
(2000)In this paper, we develop a Model-Based Geometric Control Algorithm (MGA) for controlling the dissolved oxygen in fermentation processes. The algorithm is developed on a generic system description which encompasses a wide ... -
Process engineering studies on continuous ethanol production by immobilized S. cerevisiae
(1992)Saccharomyces cerevisiae cells were immobilized on three supports—calcium alginate beads, raw and hexamethylene diamine treated bagasse—for their comparative assesment in terms of cell retention on to the carrier and ... -
Production of methionine by a multi-analogue resistant mutant of corynebacterium lilium
(2003)Multi-analogue resistant mutants of Corynebacterium lilium were developed using UV and N-methyl-N,-nitro-nitrosoguanidine (NTG) mutagenesis in order to overproduce methionine. Although the methionine yield at each stage ... -
Production of podophyllotoxin by plant cell cultures of podophyllum hexandrum in bioreactor
(2002)Submerged cultivation of Podophyllum hexandrum for the production of podophyllotoxin was carried out in a 3I stirred tank bioreactor fitted with a low-shear Setric impeller. The specific re- quirements of the medium, such ... -
Productivity enhancement in L-sorbose fermentation using oxygen vector
(2000)Sorbose, an intermediate of Vitamin-C is produced by biological oxidation of sorbitol by Acetobacter suboxydans. The utility of oxygen vector (N-hexadecane) in enhancing the sorbose accumulation and its productivity was ... -
Productivity improvement in l-sorbose biosynthesis fedbatch cultivation of gluconobacter oxydans
(2002)The effect of increased (100, 200 and 300 gl) initial sorbitol concentrations (S) was investi-gated in the sorbitol to sorbose bioconversion process. Batch cultivations with a S of 100,200 and 300 g1 were completed at 10, ... -
Protein fractionation in a vortex flow filter. I: Effect of systemhydrodynamics and solution environment on single protein transmission
(Elsevier Science, 1996)Protein fractionation by ultrafiltration has elicited considerable interest in recent years. It is now recognised that a proper choice of the membrane and/or appropriate adjustment of operating conditions can successfully ... -
Protein fractionationin a vortex flow filter. II: Separation of simulated mixtures
(Elsevier Science, 1996)This work investigates the fractionation of simulated mixtures of lysozyme/ovalbumin and lysozyme/myoglobin in a vortex flow ultrafilter. The operating parameters for the separations were identified from transmission studies ... -
Purification and characterization of two B-glucosidases from a thermo-tolerant yeast pichia etchellsii
(2003)The thermo-tolerant yeast Pichia etchellsii produced two cell-wall-bound inducible B-glucosidases, BGLI (molecular mass 186 kDa) and BGLII (molecular mass 340 kDa), which were purified by a simple, three-step method, ... -
Recent advances in microbial polyhydroxyalkanoates
(2004)Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates (HAs) synthesised by numerous bacteria as an intracellular carbon and energy storage compound. These are accumulated in the cytoplasm of cells. A number of ... -
Role of water structure on phase separation in polyelectrolyte-polyethyleneglycol based aqueous two phase systems
(2002)Partitioning of proteins in aqueous two-phase system(ATPS)has emerged as one of the important downstream processing techneques in bioprocess technology.The phase separation behavior of polyelectrolyte-polythyleneglycol(PEG)based ... -
Statistical media optimization and alkaline protease production from bacillus mojavensis in a bioreactor
(2003)Alkaline protease production in Bacillus mojavensis was improved up to 4.2-fold in a 14 l bioreactor during validation of a predicted statistical model. The final enzyme yield in the bioreactor was 2389 U m1 obtained within ... -
Studies on maintenance of vector pBK2 in Corynebacterium acetoacidophilum by co-culture experiments in a continuous bioreactor
(1996)A plasmid vector pBK2 was tested for maintenance in Corynebacterium acetoacidophilum and found to be 100% stable for 90 generations. In co-culture experiments in absence of any selection pressure it was able to cause washout ... -
Studies on phosphate uptake by acinetobacter calcoaceticus under aerobic conditions
(2004)Acinetobacter calcoaceticus was cultivated in a well-aerated stirred tank reactor and its phosphate uptake capacity was investigated. Statistical media optimization was done to figure out favourable growth conditions of ... -
Studies on protein transmission in thin channel flow module:the role of dean vortices for improving mass transfer
(2002)This study is mainly comprised of evaluating mass transfer coefficient from the volumetric flux and protein transmission data in a thin channel flow module. Dilute solutions of lysozyme were ultrafiltered through hydrophilic ... -
Surface tension of nonideal binary liquid mixtures as a function of composition
(1999)The composition dependence of the surface tension of highly nonideal organic–organic and aqueous–organic nonelectrolyte solutions is described, based on the assumption that the surface layer can be treated as a separate ...