Browsing Biochemical Engg. and Biotechnology by Title
Now showing items 14-33 of 73
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Citric acid production by solid state fermentation using sugarcane bagasse
(2003)A solid state fermentation (SSF) method was used to produce citric acid by Aspergillus niger DS 1 using sugarcane bagasse as a carrier and sucrose or molasses based medium as a moistening agent. Initially bagasse and wheat ... -
Cloning and Expression of P-Glucosidase Gene from the Yeast Pichia etchellsii
(1995)umerical Modelling of Coastal Upwelling In The Bay of Bengal Pergamon,21,(5),667-670 A 4%kilobase pairs DNA fragment from thermophilic yeast Pichia etchellsii was cloned into the vector plasmid pUC19 to form plasmid pBG55 ... -
Computer coupled substrate feeding strategies for efficient conversion of D-sorbitol to L-sorbose in fed-batch culture
(2001)L-sorbose fermentation is of industrial importance since it is an intermediate step in the industrial production of vitamin C. It involves the biological oxidation of D-sorbitol to L-sorbose using Acetobacter suboxydans. ... -
Construction of fusion vectors of corynebacteria:expression of glutathione-S-transferase fusion protein in Corynebacterium acetoacidophilum ATCC 21476
(2002)A series of fusion vectors containing glutathione-S-transferase (GST) were constructed by inserting GST fusion cassette of Escherichia coli vectors pGEX4T-1,-2 and -3 in corynebacterial vector pBK2. Efficient expression ... -
A continuous process for production of d-ribulose-1,5-bisphosphate from d-glucose
(2004)A continuous bioprocess for production of d-ribulose-1,5-bisphosphate (RuBP) using d-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow ... -
The detailed molecular mechanism of ATP synthesis in the F0 portion of ATP synthase reveals a non-chemiosmotic mode of energy coupling
(2002)Based on our torsional mechanism of ion translocation, energy transduction and energy storage in ATP synthase [Curr. Sci. 75(1998) 716]; [Curr. Sci. 77 (1999) 167]; [Curr. Sci. 78 (2000) 23]; [Biochem. Biophys. Res. Commun. ... -
Effect of cysteine on methionine production by a regulatory mutant of corynebacterium lilium
(2005)The production of methionine by submerged fermentation using a mutant strain of Corynebacterium lilium was studied to determine suitable conditions for obtaining high productivity. The mutant strain resistant to the ... -
Effect of seed culture on solid-state bioconversion of wheat straw by phanerochaete chrysosporium for animal feed production
(2002)The solid-state bioconversion of wheat straw by Phanerochaete chtysosporium for the produc-of animal feed was studied. This study was performed based on central composite experimental design.The conditions of the seed ... -
Effect of sulfur concentration on sludge acidification during the ssdml process
(1996)Heavy metals present in sewage sludges can be leached out, among other processes, by a process using a consortium of sulfur oxidizing bacteria. The metal leaching could be combined with aerobic sludge digestion to result ... -
Effects of dissolved oxygen and oxygen mass transfer on overexpression of target gene in recombinant E. coli
(1997)Overexpression of target gene (Mspl methylase) in recombinant Escherichia coli has been studied in response to dissolved oxygen and oxygen mass transfer. The rate of target gene expression attained its maximum value (2.67 ... -
Enhanced expression of the recombinant lethal factor of bacillus anthracis by fed-batch culture
(2001)High cell density cultivation has been one of the most effective ways to increase cell as well as the product yields. The structural gene for the 90-kDa lethal factor (LF) isolated from Bacillus anthracis was expressed as ... -
Expression and characterization of Pichia etchellsii B-glucosidase in Escherichia coli
(1997)The B-glucosidase enzyme is important as the terminal enzyme involved in hydrolysis of cellobiose and short-chain cellodextrins generated during enzymatic cellulose degradation. Under controlled reaction conditions the ... -
Factors limiting bacterial iron oxidation in biodesulphurization system
(2004)Batch studies have been conducted to examine the effect of various factors (which are suspected to limit the coal biodesulphurization process rate) on bacterial oxidation of ferrous iron. An attempt has been made to find ... -
Fed-batch propionic acid production by propionibacterium acidipropionici
(2000)The batch fermentations were conducted using lactose as the substrate at pH 6.5 and temperature 30C. Average batch kinetic data was eventually used to develop an unstructured mathematical model. The kinetic parameters of ... -
Immobilization of trypsin on an enteric polymer Eudragit S-100 for the biocatalysis of macromolecular substrate
(1998)Trypsin was covalently linked to Eudragit S-100 an enteric coating polymer by carbodiimide coupling method. Nearly 90% of enzyme activity was conjugated to the polymer whereas actual observed expressed activity was 64%. ... -
Improvement of gibberellic acid production using a model based fed-batch cultivation of gibberella fujikuroi
(2004)The production of gibberellic acid (GA3) by submerged cultivation of Gibberella fujikuroi was carried out in a 3l fermenter. Under optimum culture conditions, the batch culture exhibited a maximum biomass concentration of ... -
Interactions of proteins with immobilized metal ions
(2001)The design, optimization, and scale-up of a chromatographic process using immobilized metal ion affinity chromatography (IMAC)demands a thorough understanding of the fundamental factors governing the various interactions ... -
Interactions of proteins with immobilized metal ions:a comparative analysis using various isotherm models
(2001)Immobilized metal ion affinity chromatography (IMAC)is now a widely accepted technique for the purification of natural and recombinant therapeutic products and is beginning to find industrial applications. The design,optimization, ... -
Intigrated technique for pyritic sulphur reduction in indian (ASSAM) coal
(1998)Microbial desulphurization of coal using a pyrite oxidizing organism such as thiobacllus fer rooxidans result in approximately 90% pytiric sulphur reduction in 30d. An integrated technique comprised in bacterial preconditioning ... -
An investigation on glycerol biosynthesis by an osmophilic yeast in a bioreactor
(2001)Glycerol production by osmophilic yeasts has a significant potential for large-scale production. The effects of important parameters including initial glucose concentration, carbon/nitrogen (C/N) ratio, aeration rate and ...