Browsing Biochemical Engg. and Biotechnology by Title
Now showing items 21-40 of 73
-
Effect of seed culture on solid-state bioconversion of wheat straw by phanerochaete chrysosporium for animal feed production
(2002)The solid-state bioconversion of wheat straw by Phanerochaete chtysosporium for the produc-of animal feed was studied. This study was performed based on central composite experimental design.The conditions of the seed ... -
Effect of sulfur concentration on sludge acidification during the ssdml process
(1996)Heavy metals present in sewage sludges can be leached out, among other processes, by a process using a consortium of sulfur oxidizing bacteria. The metal leaching could be combined with aerobic sludge digestion to result ... -
Effects of dissolved oxygen and oxygen mass transfer on overexpression of target gene in recombinant E. coli
(1997)Overexpression of target gene (Mspl methylase) in recombinant Escherichia coli has been studied in response to dissolved oxygen and oxygen mass transfer. The rate of target gene expression attained its maximum value (2.67 ... -
Enhanced expression of the recombinant lethal factor of bacillus anthracis by fed-batch culture
(2001)High cell density cultivation has been one of the most effective ways to increase cell as well as the product yields. The structural gene for the 90-kDa lethal factor (LF) isolated from Bacillus anthracis was expressed as ... -
Expression and characterization of Pichia etchellsii B-glucosidase in Escherichia coli
(1997)The B-glucosidase enzyme is important as the terminal enzyme involved in hydrolysis of cellobiose and short-chain cellodextrins generated during enzymatic cellulose degradation. Under controlled reaction conditions the ... -
Factors limiting bacterial iron oxidation in biodesulphurization system
(2004)Batch studies have been conducted to examine the effect of various factors (which are suspected to limit the coal biodesulphurization process rate) on bacterial oxidation of ferrous iron. An attempt has been made to find ... -
Fed-batch propionic acid production by propionibacterium acidipropionici
(2000)The batch fermentations were conducted using lactose as the substrate at pH 6.5 and temperature 30C. Average batch kinetic data was eventually used to develop an unstructured mathematical model. The kinetic parameters of ... -
Immobilization of trypsin on an enteric polymer Eudragit S-100 for the biocatalysis of macromolecular substrate
(1998)Trypsin was covalently linked to Eudragit S-100 an enteric coating polymer by carbodiimide coupling method. Nearly 90% of enzyme activity was conjugated to the polymer whereas actual observed expressed activity was 64%. ... -
Improvement of gibberellic acid production using a model based fed-batch cultivation of gibberella fujikuroi
(2004)The production of gibberellic acid (GA3) by submerged cultivation of Gibberella fujikuroi was carried out in a 3l fermenter. Under optimum culture conditions, the batch culture exhibited a maximum biomass concentration of ... -
Interactions of proteins with immobilized metal ions
(2001)The design, optimization, and scale-up of a chromatographic process using immobilized metal ion affinity chromatography (IMAC)demands a thorough understanding of the fundamental factors governing the various interactions ... -
Interactions of proteins with immobilized metal ions:a comparative analysis using various isotherm models
(2001)Immobilized metal ion affinity chromatography (IMAC)is now a widely accepted technique for the purification of natural and recombinant therapeutic products and is beginning to find industrial applications. The design,optimization, ... -
Intigrated technique for pyritic sulphur reduction in indian (ASSAM) coal
(1998)Microbial desulphurization of coal using a pyrite oxidizing organism such as thiobacllus fer rooxidans result in approximately 90% pytiric sulphur reduction in 30d. An integrated technique comprised in bacterial preconditioning ... -
An investigation on glycerol biosynthesis by an osmophilic yeast in a bioreactor
(2001)Glycerol production by osmophilic yeasts has a significant potential for large-scale production. The effects of important parameters including initial glucose concentration, carbon/nitrogen (C/N) ratio, aeration rate and ... -
Kinetic model of ATP synthase: pH dependence of the rate of ATP synthesis
(2000)Abstract Recently, a novel molecular mechanism of torque generation in the F0 portion of ATP synthase was proposed [Rohatgi, Saha and Nath (1998) Curr. Sci. 75, 716-718].In this mechanism, rotation of the c-subunit was ... -
Kinetic modeling of ATP synthesis by ATP synthase and Its mechanistic implications
(2000)Based on the torsional mechanism of ATP synthesis by ATP synthase, a kinetic scheme has been developed in this work. The scheme considers adenine nucleotide transport, binding of substrates ADP and Pi, unbinding of product ... -
Microbicidal male contraceptive - risug induced morphostructural damage in e.coli
(2003)Risug, a new polymeric drug with antimicrobial properties has been shown to alter the cell morphology and structure of Escherichia coli.Topographic images, traces of surface topography and analysis of surface roughness ... -
Model-based fed-batch cultivation of R. eutropha for enhanced biopolymer production
(2004)Batch kinetics of the PHB synthesiswas established in bioreactor under controlled environmental conditions of pH and issolved oxygen. The data of the batch kinetics was used to develop and identify a mathematical model. ... -
Modeling and simulation of anaerobic startfied biofilm for mathane production and prediction of multiple steady states
(1997)The present work deal with the modeling and anaerobic metabolism in a startified biofilm consisting of two distinct layers of microorganisms in a fixed film reactor.The model is applied to steady state conditions and its ... -
Modelling the effects of electrostatic interaction with reaction-generated pH change on the kinetics of immobilized enzymes
(1995)A Mathematical model to describe the effects of electrostatic interaction with reaction-generated pH change on the kinetics of immobilized enzyme has been developed to evaluate quantitatively the effect of partitioning of ...