Browsing Biochemical Engg. and Biotechnology by Title
Now showing items 42-61 of 73
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A new approach to evaluate kinetic parameters and mass transfer coefficient in continuous stirred tank reactors.Application to antibiotic separation
(1997)A method is proposed to evaluate kinetic parameters and mass transfer cofficients for adsorption processes carried out in continuous stirred tank reactors.This method,employing a biphasic model,does not linerize nonliner ... -
A new approach to evaluate kinetic parameters and mass transfer coefficients stirred tank reactors.Application to antibiotic separation
(1997)A new method is proposed to evaluate kinetic parameters and mass transfer cofficient for adsorption processes carried out in continuous stirred tank reactors.This method,employing a biphasic mode,does not lincarize nonlinear ... -
Optimization of compactin production in chemically defined production medium by penicillium citrinum using statistical methods
(2002)Production of compactin by Penicillium citrinum was optimized using Plackett-Burman and central composite rotatable design.Out of 11 factors studied by Plackett-Burman design, six influenced the compactin production. Central ... -
Optimization of immobilized metal ion affinity chromatography for single-step purification of recombinant ovine growth hormone expressed in escherichia coli
(2003)In the present investigation, Sepharose 6B gel with 1,4-butanediol diglycidyl ether as spacer arm, iminodiacetic acid as the 21 21 ligand and Cu , Ni as metal ions were used to prepare an immobilized metal ion affinity ... -
Optimization of xylanase production by melanocarpus albomyces IIS68 in solid state fermentation using response surface methodology
(2001)Xylanase production by the thermophilic fungus, Melanocarpus albomyces IIS68, during solid state fermentation of wheat straw was studied and the effects of various variables were observed. Using the response surface ... -
Partial characterization of small plasmids from corynebacterium renale
(1995)A naphthalene-degrading strain of corynebacteria, Corynebacterium renale, harbors multiple small plasmids designated pCR1, pCR2, pCR3, and pCR4 with sizes of 1.4, 3.2, 4.4, and 5.7 kb, respectively. Plasmid pCR1 of 1.4 kb ... -
Physiological factors of growth and susceptibility to virus regulating Vero cells for optimum yield of vaccinia and cloned gene product (β-hCG)
(1994)Replication of vaccinia virus carrying β-hCG gene at the ‘tk’ locus was studied in microcarrier culture of Vero cell. The yields of recombinant vaccinia virus and its expressed product (β-hCG) were influenced by the ... -
Plasmids of corynebacteria
(1999)Corynebacteria are pleomorphic, asporogenous, Gram-positive bacteria. Included in this group are nonpathogenic soil corynebacteria, which are widely used for the industrial production of amino acids and detergents, and in ... -
A potential resource for bioconversion of domestic wastewater sludge
(2002)Twenty seven filamentous fungal strains representing five genera; Aspergillus, Penicillium, Trichoderma, Myriodontium and Pleurotus were isolated from four sources; domestic wastewater sludge cake (SC) from IWK (Indah Water ... -
Precise control of dissolved oxygen in bioreactors - a model-based geometric algorithm
(2000)In this paper, we develop a Model-Based Geometric Control Algorithm (MGA) for controlling the dissolved oxygen in fermentation processes. The algorithm is developed on a generic system description which encompasses a wide ... -
Process engineering studies on continuous ethanol production by immobilized S. cerevisiae
(1992)Saccharomyces cerevisiae cells were immobilized on three supports—calcium alginate beads, raw and hexamethylene diamine treated bagasse—for their comparative assesment in terms of cell retention on to the carrier and ... -
Production of methionine by a multi-analogue resistant mutant of corynebacterium lilium
(2003)Multi-analogue resistant mutants of Corynebacterium lilium were developed using UV and N-methyl-N,-nitro-nitrosoguanidine (NTG) mutagenesis in order to overproduce methionine. Although the methionine yield at each stage ... -
Production of podophyllotoxin by plant cell cultures of podophyllum hexandrum in bioreactor
(2002)Submerged cultivation of Podophyllum hexandrum for the production of podophyllotoxin was carried out in a 3I stirred tank bioreactor fitted with a low-shear Setric impeller. The specific re- quirements of the medium, such ... -
Productivity enhancement in L-sorbose fermentation using oxygen vector
(2000)Sorbose, an intermediate of Vitamin-C is produced by biological oxidation of sorbitol by Acetobacter suboxydans. The utility of oxygen vector (N-hexadecane) in enhancing the sorbose accumulation and its productivity was ... -
Productivity improvement in l-sorbose biosynthesis fedbatch cultivation of gluconobacter oxydans
(2002)The effect of increased (100, 200 and 300 gl) initial sorbitol concentrations (S) was investi-gated in the sorbitol to sorbose bioconversion process. Batch cultivations with a S of 100,200 and 300 g1 were completed at 10, ... -
Protein fractionation in a vortex flow filter. I: Effect of systemhydrodynamics and solution environment on single protein transmission
(Elsevier Science, 1996)Protein fractionation by ultrafiltration has elicited considerable interest in recent years. It is now recognised that a proper choice of the membrane and/or appropriate adjustment of operating conditions can successfully ... -
Protein fractionationin a vortex flow filter. II: Separation of simulated mixtures
(Elsevier Science, 1996)This work investigates the fractionation of simulated mixtures of lysozyme/ovalbumin and lysozyme/myoglobin in a vortex flow ultrafilter. The operating parameters for the separations were identified from transmission studies ... -
Purification and characterization of two B-glucosidases from a thermo-tolerant yeast pichia etchellsii
(2003)The thermo-tolerant yeast Pichia etchellsii produced two cell-wall-bound inducible B-glucosidases, BGLI (molecular mass 186 kDa) and BGLII (molecular mass 340 kDa), which were purified by a simple, three-step method, ... -
Recent advances in microbial polyhydroxyalkanoates
(2004)Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates (HAs) synthesised by numerous bacteria as an intracellular carbon and energy storage compound. These are accumulated in the cytoplasm of cells. A number of ... -
Role of water structure on phase separation in polyelectrolyte-polyethyleneglycol based aqueous two phase systems
(2002)Partitioning of proteins in aqueous two-phase system(ATPS)has emerged as one of the important downstream processing techneques in bioprocess technology.The phase separation behavior of polyelectrolyte-polythyleneglycol(PEG)based ...