Browsing Biochemical Engg. and Biotechnology by Title
Now showing items 56-73 of 73
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Productivity improvement in l-sorbose biosynthesis fedbatch cultivation of gluconobacter oxydans
(2002)The effect of increased (100, 200 and 300 gl) initial sorbitol concentrations (S) was investi-gated in the sorbitol to sorbose bioconversion process. Batch cultivations with a S of 100,200 and 300 g1 were completed at 10, ... -
Protein fractionation in a vortex flow filter. I: Effect of systemhydrodynamics and solution environment on single protein transmission
(Elsevier Science, 1996)Protein fractionation by ultrafiltration has elicited considerable interest in recent years. It is now recognised that a proper choice of the membrane and/or appropriate adjustment of operating conditions can successfully ... -
Protein fractionationin a vortex flow filter. II: Separation of simulated mixtures
(Elsevier Science, 1996)This work investigates the fractionation of simulated mixtures of lysozyme/ovalbumin and lysozyme/myoglobin in a vortex flow ultrafilter. The operating parameters for the separations were identified from transmission studies ... -
Purification and characterization of two B-glucosidases from a thermo-tolerant yeast pichia etchellsii
(2003)The thermo-tolerant yeast Pichia etchellsii produced two cell-wall-bound inducible B-glucosidases, BGLI (molecular mass 186 kDa) and BGLII (molecular mass 340 kDa), which were purified by a simple, three-step method, ... -
Recent advances in microbial polyhydroxyalkanoates
(2004)Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates (HAs) synthesised by numerous bacteria as an intracellular carbon and energy storage compound. These are accumulated in the cytoplasm of cells. A number of ... -
Role of water structure on phase separation in polyelectrolyte-polyethyleneglycol based aqueous two phase systems
(2002)Partitioning of proteins in aqueous two-phase system(ATPS)has emerged as one of the important downstream processing techneques in bioprocess technology.The phase separation behavior of polyelectrolyte-polythyleneglycol(PEG)based ... -
Statistical media optimization and alkaline protease production from bacillus mojavensis in a bioreactor
(2003)Alkaline protease production in Bacillus mojavensis was improved up to 4.2-fold in a 14 l bioreactor during validation of a predicted statistical model. The final enzyme yield in the bioreactor was 2389 U m1 obtained within ... -
Studies on maintenance of vector pBK2 in Corynebacterium acetoacidophilum by co-culture experiments in a continuous bioreactor
(1996)A plasmid vector pBK2 was tested for maintenance in Corynebacterium acetoacidophilum and found to be 100% stable for 90 generations. In co-culture experiments in absence of any selection pressure it was able to cause washout ... -
Studies on phosphate uptake by acinetobacter calcoaceticus under aerobic conditions
(2004)Acinetobacter calcoaceticus was cultivated in a well-aerated stirred tank reactor and its phosphate uptake capacity was investigated. Statistical media optimization was done to figure out favourable growth conditions of ... -
Studies on protein transmission in thin channel flow module:the role of dean vortices for improving mass transfer
(2002)This study is mainly comprised of evaluating mass transfer coefficient from the volumetric flux and protein transmission data in a thin channel flow module. Dilute solutions of lysozyme were ultrafiltered through hydrophilic ... -
Surface tension of nonideal binary liquid mixtures as a function of composition
(1999)The composition dependence of the surface tension of highly nonideal organic–organic and aqueous–organic nonelectrolyte solutions is described, based on the assumption that the surface layer can be treated as a separate ... -
Synthesis of kyotorphin precursor by an organic solvent-stable protease from bacillus licheniformis RSP-09-37
(2004)The synthesis of the analgesic dipeptide kyotorphin precursor (Bz-Tyr-Arg-NH2) was studied under kinetically controlled conditions in 10–90% (v/v) aqueous-acetonitrile media at −20 ◦C using a novel protease obtained from ... -
Thermal stability of alcohol dehydrogenase enzyme determined by activity assay and calorimetry
(1998)The thermostability of pure yeast alcohol dehydrogenase was investigated at various temperatures, in the presence and absence of sucrose, by both activity assay and differential scanning calorimetry. The thermal inactivation ... -
The torsional mechanism of energy transduction and ATP synthesis as a breakthrough in our understanding of the mechanistic, kinetic and thermodynamic details
(2004)Novel insights into mechanistic, kinetic and thermodynamic details of ATP synthesis by F1F0-ATP synthase in oxidative phosphorylation and photophosphorylation have been offered from the perspective of the torsional mechanism ... -
Updated mathematical model and fed-batch strategies for poly-B-hydroxybutyrate (PHB) production by alcaligenes eutrophus
(1998)The growth and the intracellular biopolymer Poly-B Hydroxybutyrate (PHB) storage kinetics of Alcaligenes eutrophus were studied in a batch cultivation process.N/C ratio was found to both limit and inhibit the growth of the ... -
Utilisation of fruits waste for citric acid production by solid state fermentation
(2003)A solid state fermentation method was used to utilise pineapple, mixed fruit and maosmi waste as substrates for citric acid production using Aspergillus niger DS 1. Experiments were carried out in the presence and absence ... -
Xanthan Biosynthesis in Continuous Culture: Citric Acid
(1995)Role of citric acid on the biosynthesis of xanthan was studied in continuous culture. Increased citric acid consumption resulted in an increase in ATP production by cells which was used in the synthesis of xanthan. Mass ... -
Xylanase production by a hyperxylanolytic mutant of Fusarium oxysporum
(1995)Mutagensis of Fusarium Oxysporum DSM 841 Enhanced the activity of xyclanase and Beta-xylanace enzymes by more than threefold. Wild-Strain spores were subjected to UV orN-methyl-N'-nitro-N-nitrosoguanidine Treatments. The ...