Browsing Biochemical Engg. and Biotechnology by Issue Date
Now showing items 1-20 of 73
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Process engineering studies on continuous ethanol production by immobilized S. cerevisiae
(1992)Saccharomyces cerevisiae cells were immobilized on three supports—calcium alginate beads, raw and hexamethylene diamine treated bagasse—for their comparative assesment in terms of cell retention on to the carrier and ... -
Physiological factors of growth and susceptibility to virus regulating Vero cells for optimum yield of vaccinia and cloned gene product (β-hCG)
(1994)Replication of vaccinia virus carrying β-hCG gene at the ‘tk’ locus was studied in microcarrier culture of Vero cell. The yields of recombinant vaccinia virus and its expressed product (β-hCG) were influenced by the ... -
Cloning and Expression of P-Glucosidase Gene from the Yeast Pichia etchellsii
(1995)umerical Modelling of Coastal Upwelling In The Bay of Bengal Pergamon,21,(5),667-670 A 4%kilobase pairs DNA fragment from thermophilic yeast Pichia etchellsii was cloned into the vector plasmid pUC19 to form plasmid pBG55 ... -
Xanthan Biosynthesis in Continuous Culture: Citric Acid
(1995)Role of citric acid on the biosynthesis of xanthan was studied in continuous culture. Increased citric acid consumption resulted in an increase in ATP production by cells which was used in the synthesis of xanthan. Mass ... -
Modelling the effects of electrostatic interaction with reaction-generated pH change on the kinetics of immobilized enzymes
(1995)A Mathematical model to describe the effects of electrostatic interaction with reaction-generated pH change on the kinetics of immobilized enzyme has been developed to evaluate quantitatively the effect of partitioning of ... -
Partial characterization of small plasmids from corynebacterium renale
(1995)A naphthalene-degrading strain of corynebacteria, Corynebacterium renale, harbors multiple small plasmids designated pCR1, pCR2, pCR3, and pCR4 with sizes of 1.4, 3.2, 4.4, and 5.7 kb, respectively. Plasmid pCR1 of 1.4 kb ... -
Xylanase production by a hyperxylanolytic mutant of Fusarium oxysporum
(1995)Mutagensis of Fusarium Oxysporum DSM 841 Enhanced the activity of xyclanase and Beta-xylanace enzymes by more than threefold. Wild-Strain spores were subjected to UV orN-methyl-N'-nitro-N-nitrosoguanidine Treatments. The ... -
Protein fractionationin a vortex flow filter. II: Separation of simulated mixtures
(Elsevier Science, 1996)This work investigates the fractionation of simulated mixtures of lysozyme/ovalbumin and lysozyme/myoglobin in a vortex flow ultrafilter. The operating parameters for the separations were identified from transmission studies ... -
Studies on maintenance of vector pBK2 in Corynebacterium acetoacidophilum by co-culture experiments in a continuous bioreactor
(1996)A plasmid vector pBK2 was tested for maintenance in Corynebacterium acetoacidophilum and found to be 100% stable for 90 generations. In co-culture experiments in absence of any selection pressure it was able to cause washout ... -
Effect of sulfur concentration on sludge acidification during the ssdml process
(1996)Heavy metals present in sewage sludges can be leached out, among other processes, by a process using a consortium of sulfur oxidizing bacteria. The metal leaching could be combined with aerobic sludge digestion to result ... -
Protein fractionation in a vortex flow filter. I: Effect of systemhydrodynamics and solution environment on single protein transmission
(Elsevier Science, 1996)Protein fractionation by ultrafiltration has elicited considerable interest in recent years. It is now recognised that a proper choice of the membrane and/or appropriate adjustment of operating conditions can successfully ... -
Adsorption characteristics of cellulases from a constitutive mutant of trichoderma reesei
(1997)The adsorption parameter, A,, (maximum protein adsorbed/g substrate) and KA (adsorption equilibrium constant) of Trichoderma reesei C-5 cellulases were 58.8 mg protein/g cellulose and 11.6 x 104 Z/m01 respec-tively. The ... -
Modeling and simulation of anaerobic startfied biofilm for mathane production and prediction of multiple steady states
(1997)The present work deal with the modeling and anaerobic metabolism in a startified biofilm consisting of two distinct layers of microorganisms in a fixed film reactor.The model is applied to steady state conditions and its ... -
Effects of dissolved oxygen and oxygen mass transfer on overexpression of target gene in recombinant E. coli
(1997)Overexpression of target gene (Mspl methylase) in recombinant Escherichia coli has been studied in response to dissolved oxygen and oxygen mass transfer. The rate of target gene expression attained its maximum value (2.67 ... -
Biosynthesis of xylanolytic and xylan-debranching enzymes in Melanocarpus albomyces IIS 68
(1997)Melanocarpus albomyces IIS 68 synthesizes xylanase, ,&xylosidase and xylan debranching enzymes, namely, acetyl esterase, a+arabinofuranosidase and a-o-giucuronidase, when grown on various substrates. All five enzymes were ... -
A new approach to evaluate kinetic parameters and mass transfer coefficients stirred tank reactors.Application to antibiotic separation
(1997)A new method is proposed to evaluate kinetic parameters and mass transfer cofficient for adsorption processes carried out in continuous stirred tank reactors.This method,employing a biphasic mode,does not lincarize nonlinear ... -
Analysis of autocatalytic reactions with michaelis-menten kinetics in an isothermal continuous stirred tank reactor
(1997)A systematic analysis of the behavior of autocatalytic reactions obeying Michaelis-Menten kinetics and taking place in an isothermal continuous stirred tank reactor is presented. The mathematical model is solved for general ... -
A new approach to evaluate kinetic parameters and mass transfer coefficient in continuous stirred tank reactors.Application to antibiotic separation
(1997)A method is proposed to evaluate kinetic parameters and mass transfer cofficients for adsorption processes carried out in continuous stirred tank reactors.This method,employing a biphasic model,does not linerize nonliner ... -
Expression and characterization of Pichia etchellsii B-glucosidase in Escherichia coli
(1997)The B-glucosidase enzyme is important as the terminal enzyme involved in hydrolysis of cellobiose and short-chain cellodextrins generated during enzymatic cellulose degradation. Under controlled reaction conditions the ... -
Intigrated technique for pyritic sulphur reduction in indian (ASSAM) coal
(1998)Microbial desulphurization of coal using a pyrite oxidizing organism such as thiobacllus fer rooxidans result in approximately 90% pytiric sulphur reduction in 30d. An integrated technique comprised in bacterial preconditioning ...